A DEFIANT Boris Johnson has said he will not negotiate a fresh Brexit delay with the EU despite losing a key Commons vote.

At a special Saturday sitting, MPs voted by 322 to 306 in favour an amendment withholding approval of his Brexit deal until legislation to implement it is in place.

The amendment tabled by former Cabinet minister Sir Oliver Letwin was intended to force him to comply with the so-called Benn Act requiring him to seek a Brexit extension.

But amid noisy Commons scenes, Mr Johnson insisted that he was not “daunted or dismayed” by the result and remained committed to taking Britain out by October 31.

“I will not negotiate a delay with the EU, neither does the law compel me to do so,” he said.

Downing Street refused to offer any explanation as to why the Prime Minister did not consider he was obliged to negotiate a fresh amendment.

Asked if previous statements from ministers that the Government would comply with the law still stood, the Prime Minister’s official spokesman told a Westminster briefing: “Governments comply with the law.”

Labour leader Jeremy Corbyn warned Mr Johnson that he could not ignore the provisions of the Benn Act.

“It’s an emphatic decision by this House that has declined to back the Prime Minister’s deal today and clearly voted to stop a no-deal crash-out from the European Union,” he said.

“The Prime Minister must now comply with the law. He can no longer use the threat of a no-deal crash-out to blackmail members to support his sell-out deal.”

The SNP leader at Westminster Ian Blackford said that if Mr Johnson acted as if we was “above the law”, he would find himself in court.

Liberal Democrat leader Jo Swinson said: “The most urgent thing right now is the Prime Minister complies with the law.”

The European Commissions’ chief spokeswoman Mina Andreeva urged the Government “to inform us about the next steps as soon as possible”.

The Benn Act sets a deadline of 11 pm on Saturday for the Prime Minister to get a deal if the UK is to leave on October 31, otherwise he is supposed to seek an extension.

Commons Leader Jacob Rees-Mogg said the Government was planning to give MPs a fresh chance to have a “meaningful vote” on the deal hammered out by Mr Johnson with Brussels on Monday.

The voting took place as tens of thousands of protesters converged on central London in People’s Vote campaign march in support of a second referendum.

The result was greeted by loud cheers by the crowd assembled outside the Palace of Westminster in Parliament Square.

The Government was consigned to defeat after the 10 MPs of Mr Johnson’s erstwhile allies in the DUP joined the other opposition parties in voting the amendment.

The party is furious about the proposed customs arrangements for Northern Ireland which would see checks on good coming from the rest of the UK.

Ten former Conservative MPs who now sit as independents, including former cabinet ministers Ken Clarke, Philip Hammond and Amber Rudd, also backed the amendment.

In contrast just six Labour rebels voted against it.

Following the vote Mr Johnson said that he did not believe the other EU member states would be “attracted” to a further delay.

He said the Government would be tabling legislation next week to implement the terms of the agreement.

“I hope that then Honourable Members, faced with a choice of our new deal for the UK and the European Union, will change their minds because it was pretty close today,” he said.

“I hope that they will change their minds and support this deal in overwhelming numbers.

“I will continue to do all I can to get Brexit done on October 31.”